Assay of lobetyolin and atractylenoide III in Radix Codonopsis from different habitats

Objective To determine the contents of lobetyolin and atractylenoide Ⅲ in Radix Codonopsis from different habitats,and to explore the effect of environment on the chemical constituents of Radix Codonopsis.Methods The contents of lobetyolin and atractylenolide Ⅲ in the Radix Condonopsis cultivated in the same habit but from different original habit were detected by HPLC.Results Under the chosen conditions,there were good linear relationships between the input quantity and the peak areas of lobetyolin and atractylenoide Ⅲ in the ranges of 0.10-1.6 μg(r=0.999 4)and 0.01-2.0 μg(r=0.999 9),respectively.The mean recovery rates of lobetyolin and atractylenoide Ⅲ were 103.0% and 100%,respectively.RSD were 0.02%(n=6)and 2.1%(n=6),respectively.The contents of lobetyolin and atractylenoide Ⅲ showed significant difference from different original habitats.The contents of lobetyolin and atractylenolide Ⅲ in the Radix Codonopsis from different original habitats showed significant changes after being introduced.Conclusion Environmental conditions have a significant influence on the chemical constituents of Radix Codonopsis.The natural environmental conditions of Shanxi are suitable for the cultivation and introduction.