EMS mutagenesis and ALS- inhibitor herbicide- resistant mutants of Brassica napus L.

To enhance rapeseed germplasm and improve rapeseed variety with resistance to tribenuron- methyl,seeds of Zhongshuang 9( Brassica napus) were treated with 0. 4%,0. 8%,1. 0% and 1. 2% of EMS( ethyl methansulfonate) solution respectively. Results showed that( 1) 1. 0% of EMS was optional for mutagenesis and the phenotype variation in four M2populations were 11. 26%,14. 82%,27. 19% and 12. 38% respectively.( 2) Different concentrations of EMS had different effects on organs.( 3) Three mutants with resistance to tribenuron-methyl were with mutation frequency about 0. 000 1. The M2mutant library,including mutants of cotyledons,leaves,flowers,plant- types,siliques,was available for genetic studies and breeding in rapeseed.