Analysis of Monosaccharides and Uronic Acids in Fucoidon from Seaweed by Pre-column Derivatization High Performance Liquid Chromatography

High performance liquid chromatography(HPLC) was employed for the analysis of monosaccharide and uronic acids in fucoidon from fresh seaweed with pre-column derivatization.After degradated by 4 mol/L trifluoroacetic acid and derivatived with 1-phenyl-3-methyl-5-pyrazolone(PMP),monosaccharides and uronic acids in fucoidon were separated by reversed-phase HPLC with gradient elution,and monitored by ultraviolet detector at 250 nm.The result show that fucoidon consists of 5 kinds of monosaccharides and 2 kinds of uronic acids.The ratio of mannose,rhamnose,glucuronic acid,galacturonic acid,glucose,galactoses,fucose is 0.03∶3.7∶0.22∶0.74∶0.12∶1.1∶ 4.1.The developed method is suitable for the rapid analysis of polysaccharide with good reproducibility.