The Protective Effects of Xiaoshuan Oral Liquid on Myocardial Ischemia-reperfusion Injury in Rats

Objective To investigate the protective effect and related mechanisms of Xiaoshuan oral liquid (XSOL) aganist myocardial ischemia-reperfusion injury in rats. Methods Thirth-two male Sprague -Dawley rats were randomly divided into four groups, the sham -operation group, ischemia - reperfusion group, XSOL group, and dilthiazem group. Myocardial ischemia-reperfusion injury model was made by ligating and recanalizing the left coronary artery. Electrocardiogram was continuously traced to observe the arrhythmia caused by reperfusion. The content of malondialdehyde (MDA) and superoxide dismutase (SOD) in myocardium tissue and the activity of creatine phosphokinase (CK) and lactate dehydrogenase (LDH) in serum were measured. The expressions of bcl-2 and bax gene were observed by RT-PCR. Results Compared with the ischemia-reperfusion group, XSOL decreased the incidence of arrhythmia, improved the activity of SOD and decreased the content of MDA, CK and LDH. The expression of bcl-2 was enhanced, while bax weakened when XSOL was adopted. Conclusion XSOL exerts a protective effect on myocardial ischemia-reperfusion injury in rats. The mechanism may relate to relieving the injury caused by oxygen free radical and repressing the myocyte apoptosis.