SOX2
生物
祖细胞
细胞生物学
鲜味
Wnt信号通路
祖细胞
味觉感受器
味蕾
品味
刺猬
干细胞
信号转导
转录因子
遗传学
生物化学
基因
作者
Lauren A. Shechtman,Joseph Scott,Eric Stanley Larson,Trevor J Isner,B. J. Johnson,David Gaillard,Peter J. Dempsey,Linda A. Barlow
出处
期刊:Development
[The Company of Biologists]
日期:2023-02-15
卷期号:150 (4)
被引量:3
摘要
ABSTRACT Taste buds on the tongue contain taste receptor cells (TRCs) that detect sweet, sour, salty, umami and bitter stimuli. Like non-taste lingual epithelium, TRCs are renewed from basal keratinocytes, many of which express the transcription factor SOX2. Genetic lineage tracing has shown that SOX2+ lingual progenitors give rise to both taste and non-taste lingual epithelium in the posterior circumvallate taste papilla (CVP) of mice. However, SOX2 is variably expressed among CVP epithelial cells, suggesting that their progenitor potential may vary. Using transcriptome analysis and organoid technology, we show that cells expressing SOX2 at higher levels are taste-competent progenitors that give rise to organoids comprising both TRCs and lingual epithelium. Conversely, organoids derived from progenitors that express SOX2 at lower levels are composed entirely of non-taste cells. Hedgehog and WNT/β-catenin are required for taste homeostasis in adult mice. However, manipulation of hedgehog signaling in organoids has no impact on TRC differentiation or progenitor proliferation. By contrast, WNT/β-catenin promotes TRC differentiation in vitro in organoids derived from higher but not low SOX2+ expressing progenitors.
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