Interaction of Vitamin B 12 With Soybean Lipophilic Proteins Co-Modified by Ph-Shifting and High-Pressure Homogenization: Focus on Structure and Physicochemical Properties

This study focuses on the modification of soybean lipophilic protein (SLP) through pH-shifting and high-pressure homogenization, aiming to elucidate the impact of the combined modification on the dissociation and aggregation behavior of SLP. Simultaneously, modified SLP was employed as a carrier for vitamin B12, exploring the influence of their interaction on physicochemical properties of composite system. Results indicate that, under co-modification at pH 11 and high homogenization pressure of 150 Mpa, the particle size of SLP diminished to 368 nm, while the absolute value of zeta potential gradually increased to 26.97 mV. Molecular docking analysis confirmed the successful loading of vitamin B12 onto SLP, demonstrating high loading rate. In vitro digestion assessments reveal that complexes prepared with modified SLP enhance the digestive properties of vitamin B12. This study not only broadens the repertoire of nanoparticle preparation methods but also establishes a theoretical foundation for the formulation of SLP-vitamin B12 complexes.