Dopamine-Modulated Highly Oxygen-Vacancy Ce Metal–Organic Frameworks Boost Phosphatase-Catalyzed Chemiluminescence

= 1428.57 μM). The experimental data and density functional theory calculations demonstrated that the flexible scaffold coating of PDA endowed Ce-2-MIm with improved oxygen vacancies for the preferable binding affinity and hydrolysis activity. The marked affinity effect of the Ce-2-MIm-based support imparted the dominant improvement of catalytic activity to fixed enzymes in the enzyme immobilization. For the proof-of-concept application on chemiluminescence (CL) catalysis, the prepared Ce-2-MIm@ALP@PDA is successfully employed for the detection of carbendazol (CAB), with a low detection limit of 56 pg/mL. This work highlights the merits of nanozymes as an efficient affinity-type support for enzyme assemblies, which exhibits great potential for considerable activity exaltation of enzymes even in an immobilization manner.