Correlation between Hydrogen Bond Strength and Temperature: A Quantitative Single-Molecule Study over a Broad Temperature Range

* = 7.88 - 1.34ln(T - 251.64). This formula enables the prediction of H-bond intrinsic strength at various temperatures within a reasonable range, which provides insights into the precise control of H-bond strength through temperature regulation. Although the formula may not be universally applicable, this pioneering work represents an upgrade in our understanding of this fundamental relationship from a qualitative to a quantitative perspective.