生物
内含子
RNA剪接
细胞生物学
卵母细胞
遗传学
核糖核酸
选择性拼接
信使核糖核酸
基因
分子生物学
胚胎
作者
Hua Zhang,Jun-Chao Jiang,Yun-Wen Wu,Yuansong Yu,Huanan Wang,Nai-Zheng Ding,Heng-Yu Fan
出处
期刊:Development
[The Company of Biologists]
日期:2022-09-30
摘要
Recent large-scale mRNA sequencing has shown that introns are retained in 5-10% of mRNA, and these events are named intron retention (IR). IR has been recognized as a key mechanism in the regulation of gene expression. However, the role of this mechanism in female reproduction in mammals remains unclear. RNA terminal phosphate cyclase B (RTCB) is an RNA ligase and we found that RTCB conditional knockout mice have premature ovarian failure and that RTCB plays a crucial role in follicular development. RTCB regulated the splicing of transcripts related to DNA methylation and DNA damage repair. In addition, it regulated the resumption of oocyte meiosis by affecting CDK1 activation. Moreover, the loss of RTCB suppressed zygotic genome activation (ZGA) and decreased translation at the global level. In addition, Rtcb deletion resulted in the accumulation of maternal mRNAs containing unspliced introns and a decline in the overall level of transcripts. As a result, the Rtcb-/- females were sterile. Our study highlights the important role of RTCB-regulated noncanonical alternative splicing in female reproduction.
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