Optimization of solvent extraction conditions for total carotenoids in rapeseed using response surface methodology

The optimum total carotenoids (TC) extraction from rapeseed with solvent extraction method by UV-visible spectrophotometer determination was investigated by using response surface methodology (RSM).Extraction duration, repeated extraction cycles, solvent-solid ratio and extraction temperature were assumed to be the most important factors affecting solvent extraction for the determination of TC.Optimum solvent extraction conditions for maximizing the determination of TC were: extraction duration 7.3h, repeated extraction three times, ratio of solvent-solid (v/w, mL/mg) 29:1, extraction temperature 42°C.Under the optimal conditions, the yield of TC was up to 4.79 mg /100g.The model had a satisfactory coefficient of R 2 (= 0.912) and verified experimentally.The results showed that the conditions were mild and useful for maximizing a quantitative spectrophotometer determination of TC in rapeseed.