琼脂糖
DNA
核酸凝胶电泳
琼脂糖凝胶电泳
色谱法
移液管
凝胶电泳
化学
电泳
DNA提取
简单(哲学)
计算机科学
聚合酶链反应
生物化学
哲学
物理化学
认识论
基因
作者
Fan Chang-fa,Xingguo Mei
摘要
A simple method of recovering DNA from agarose gel that is fast, inexpensive, and friendly both to operators and environment is described. Two rows of wells are made in an agarose gel, and a DNA sample is loaded into the well nearest to the negative pole for separation by electrophoresis. Recovery is accomplished by pipetting the DNA-containing TAE buffer from the well near the positive pole after target DNA fragments have migrated into the well. A recovery rate of up to 94 +/- 2.3% was observed with this method.
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