The gastrodin in Gastrodia elata Bl. was analyzed with HPLC. The samples were separated with the column of Hypersil ODS-2 in acetonitrile-0.05% phosphoric acid(3∶97,V / V). The results showed that gastrodin in Gastrodia elata Bl. f.glaucca S Chow, Wuhong hybrid and G. elata Bl. f elata was 0.423%(m / m), 0.321%, and 0.225%, respectively. The gastrodin content in Wuhong hybrid from the place of low altitude in Dabie mountain was found to be higher compared with that in G. elata Bl. f elata.