化学
脂多糖
肿瘤坏死因子α
炎症
格里斯试验
酶
促炎细胞因子
消炎药
分子生物学
药理学
生物化学
免疫学
亚硝酸盐
生物
有机化学
硝酸盐
作者
Luye Zong,Jin Zhang,Liping Dai,Jian Liu,Yan Yang,Jiankun Xie,Xiangdong Luo
标识
DOI:10.1002/cbdv.202000477
摘要
Abstract Rhododendron molle G.Don is a well‐known traditional medicine which has been used to treat rheumatic inflammation. In this study, an inflammatory model of lipopolysaccharide (LPS)‐stimulated RAW264.7 cells was established to analyze the anti‐inflammatory effect and potential mechanism of the methanol extract of R. molle leaves (RLE). The production of NO and the expression of tumor necrosis factor by LPS were detected by Griess reaction and enzyme linked immunosorbent assay (ELISA). The mRNA expression of TNF‐α , IL‐1β , IL‐6 , COX‐2 and iNOS was measured by qRT‐PCR assay. Griess and qRT‐PCR showed that the RLE could significantly concentration‐dependently inhibit NO production and the expression of many pro‐inflammatory cytokines and inflammatory‐related enzymes. Scanning electron microscope (SEM) analysis indicated that RLE could inhibit LPS‐stimulated RAW264.7 macrophages activation. The protein level of TNF‐ α and IL‐1 β were decreased over 50 % at 100 μg/ml of RLE, as detected by ELISA. These results indicated that RLE had strong anti‐inflammatory and immunomodulatory activity.
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