Preparation of Magnetic Molecularly Imprinted Polymer Fe3O4@SiO2@Ag-MIPs and Its Identification and SERS Detection of Ofloxacin

In this paper, magnetic substrate, molecular imprinting technology (MIT) and surface-enhanced Raman scattering (SERS) technology were combined to prepare core-shell magnetic molecularly imprinted polymer Fe3O4@SiO2@Ag-MIPs for quantitative detection of ofloxacin. The results showed that the concentration of ofloxacin within the range of 10−3–10−8 mol/L showed a good linear relationship with the Raman peak intensity at 1416 cm−1. The prepared molecularly imprinted polymer had excellent uniformity and dispersibility. Raman testing of commercially available ofloxacin eye drops showed good recovery, indicated that it can be used for the quantitative detection of actual samples. The Fe3O4@SiO2 particles were prepared by hydrothermal method, and then the surface was coated with silver particles by seeding. Fe3O4@SiO2@Ag-MIPs molecularly imprinted polymer was prepared using silylation reagent as crosslinker and OFX as a template molecule, and used for SERS detection.