纳米晶
光致发光
量子产额
荧光
量子点
锗
纳米技术
材料科学
水溶液
生物传感器
细胞内
生物物理学
化学工程
化学
生物化学
有机化学
光电子学
硅
生物
工程类
物理
量子力学
作者
Feng Li,Jing Wang,Shuqing Sun,Hai Wang,Zhiyong Tang,Guangjun Nie
出处
期刊:Small
[Wiley]
日期:2015-01-12
卷期号:11 (16): 1954-1961
被引量:34
标识
DOI:10.1002/smll.201402743
摘要
A green-light emitting germanium nanocrystal-based biosensor to monitor lysosomal pH changes is developed. The Ge nanocrystals are synthesized in an aqueous solution with a significantly enhanced photoluminescence quantum yield of 26%. This synthesis involves a facile solution based route which avoided the use of toxic or environmentally unfriendly agents. Importantly, the photoluminescence intensity of the synthesized Ge nanocrystals is particularly sensitive to changes in pH between 5 and 6. When incubated with cultured cells, the nanocrystals are internalized and subsequently translocated via the lysosomal pathway, and the Ge nanocrystals' fluorescence are greatly enhanced, even when the lysosomal pH is only slightly increased. These results reveal that the Ge nanocrystals possess high pH sensitivity compared to a commercially available dye, LysoSensor Green DND-189. The fluorescent properties of the Ge nanocrystals are demonstrated to be dependent on both the crystal form and their surface chemistry. The superior fluorescence properties and bioapplicability of the Ge nanocrystals makes them a promising intracellular bioimaging probe for monitoring various pH-sensitive processes in cells.
科研通智能强力驱动
Strongly Powered by AbleSci AI