生物
分子生物学
互补DNA
跨膜蛋白
肥大细胞
打开阅读框
表位
基因
免疫沉淀
肽序列
抗体
生物化学
遗传学
免疫学
受体
作者
Kang Li,Shen Wu Wang,Yucheng Liu,Rachel E. Martin,Lixin Li,Meisheng Lu,Salah-Eddine Lamhamedi-Cherradi,Guanghui Hu,Sossiena Demissie-Sanders,Jie Zheng,Fan Chung,Thomas Oates,Zhengbin Yao
出处
期刊:Genomics
[Elsevier]
日期:2005-07-01
卷期号:86 (1): 68-75
被引量:33
标识
DOI:10.1016/j.ygeno.2005.03.006
摘要
A cDNA encoding a new type II transmembrane protein has been isolated from human mast cells by subtraction cloning. This cDNA contains an open reading frame of 186 amino acids. RT-PCR analysis showed that this gene is differentially expressed in mast cells. Therefore, the peptide encoded by this gene was termed mast cell-expressed membrane protein 1 (MCEMP1). The MCEMP1 gene contains seven exons and was mapped to human chromosome 19p13.3. The epitope-tagged MCEMP1 has been expressed in mammalian cells and found to be localized to the cellular membrane with its C-terminus extending to the outside of the membrane and N-terminus into the cytoplasmic compartment. Monoclonal antibodies against MCEMP1 were generated and characterized by immunoprecipitation and FACS. The results showed that the native MCEMP1 is expressed in cord blood-derived mast cells and HMC-1 and THP-1 cell lines, but not in other cell types that we have tested. Immunochemical staining of human lung sections showed that MCEMP1 staining is specifically associated with lung mast cells.
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