MATRIX SOLID PHASE DISPERSION FOR THE EXTRACTION OF BISPHENOL-A FROM HUMAN BREAST MILK PRIOR TO HPLC ANALYSIS

Matrix Solid Phase Dispersion was applied to the effective and quantitative extraction of Bisphenol A (4,4′-(propane-2,2-diyl)diphenol) from human breast milk. A reversed phase high performance liquid chromatographic method was further developed and validated. The mobile phase consisted of CH3CN-H2O (70:30, v/v) and was delivered isocratically. The analytical column was Hichrom Kromasil 100-5C18 (250 × 4.6 mm, 5 µm) and was operated at ambient temperature. UV detection was performed at 230 nm. Sample preparation was achieved with matrix solid phase dispersion (MSPD) using the sorbent of Lichrolut cartridges with high recoveries (95.5%). The detection limit of the method was 5.3 µg/kg. Triethylene glycol dimethacrylate (TEGDMA) was used as internal standard. Validation of the method was performed in terms of accuracy and precision: within-day assay (n = 6) and between-day assay (n = 3 × 3) and was found to be satisfactory, with high accuracy and precision results. The developed method was comparatively applied to the analysis of commercial cow milk samples with success.