极化活动区
肢芽
音猬因子
生物
顶外胚层嵴
肢体发育
刺猬
解剖
细胞生物学
维甲酸
外胚层
基因
胚胎
胚胎发生
遗传学
信号转导
作者
Robert D. Riddle,Randy L. Johnson,Ed Laufer,Cliff Tabin
出处
期刊:Cell
[Elsevier]
日期:1993-12-01
卷期号:75 (7): 1401-1416
被引量:2223
标识
DOI:10.1016/0092-8674(93)90626-2
摘要
The zone of polarizing activity (ZPA) is a region at the posterior margin of the limb bud that induces mirror-image duplications when grafted to the anterior of a second limb. We have isolated a vertebrate gene, Sonic hedgehog, related to the Drosophila segment polarity gene hedgehog, which is expressed specifically in the ZPA and in other regions of the embryo, that is capable of polarizing limbs in grafting experiments. Retinoic acid, which can convert anterior limb bud tissue into tissue with polarizing activity, concomitantly induces Sonic hedgehog expression in the anterior limb bud. Implanting cells that express Sonic hedgehog into anterior limb buds is sufficient to cause ZPA-like limb duplications. Like the ZPA, Sonic hedgehog expression leads to the activation of Hox genes. Sonic hedgehog thus appears to function as the signal for antero-posterior patterning in the limb.
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