N-乙酰氨基葡萄糖
尿苷
核苷酸
乙酰化
化学
乙酰氨基葡萄糖
薄层色谱法
酶
色谱法
纸色谱法
生物化学
糖
核糖核酸
基因
作者
Les Lang,Stuart Kornfeld
标识
DOI:10.1016/0003-2697(84)90163-5
摘要
A simplified procedure for the synthesis of [β-32P]UDP-N-acetylglucosamine is described. This novel method utilizes commericially available enzymes, chemical acetylation, and preparative thin-layer chromatography and can be used to synthesize millicurie quantities of the sugar nucleotide at an extremely high specific activity and purity.
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