细胞周期蛋白依赖激酶
发芽
生物
细胞周期蛋白
激酶
细胞周期蛋白依赖激酶2
蛋白激酶A
细胞生物学
细胞周期蛋白依赖激酶复合物
分子生物学
生物化学
细胞周期
基因
植物
作者
Natividad de Jesús Juárez,Alfredo Mancilla,Elpidio García,Jorge M. Vázquez‐Ramos
出处
期刊:Seed Science Research
[Cambridge University Press]
日期:2008-06-01
卷期号:18 (2): 67-75
被引量:5
标识
DOI:10.1017/s0960258508975048
摘要
Abstract Plant KRP proteins are cyclin/cyclin-dependent kinase subunit (Cdk) inhibitors that share a limited homology with mammalian p27 Kip1 proteins. Several KRPs have been reported in maize ( Zea mays L.), of which Zeama;KRP1 was studied during maize germination. Expression of the Zeama;KRP1 gene did not vary during the 24 h germination period. A homologous antibody raised against the 13 kDa carboxy end of the Zeama;KRP1 polypeptide, a sequence containing the cyclin/Cdk inhibitory domain, indicated the existence of a 22 kDa protein in maize embryonic axes, the amount of which also remained unchanged during germination. Neither abscisic acid nor cytokinins modified the amount of protein. The purified Zeama;KRP1 polypeptide inhibited the kinase activity associated with Zeama;PCNA and Zeama;CycD2;1, and also the kinase activity in p13 Suc1 -pulled-down complexes. However, there were differences in the inhibition pattern during germination. Whereas kinase activity in proliferating cell nuclear antigen (PCNA) or CycD2;1 immunoprecipitates was strongly inhibited mainly during early germination, that in p13 Suc1 -pulled-down complexes was mainly inhibited at late times, suggesting that each protein complex is composed of different cyclins and/or Cdks.
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