A Study on Antibacterial Activity of Antibacterial Peptide From Lucilia sericata After Treatment at High Temperature

Objective To investigate the stability of antibacterial activity of antibacterial peptide from Lucilia sericata after treatment at high temperature for different time. Methods After being bathed at 100 ℃, 80 ℃ and 60 ℃ for 0 sec, 30 sec, 1 min, 3 min and 5 min, the immunized hemolymph were test against Micrococcus lysodeikticus respectively, the bacteriostatic effects were observed after 24 hs and the diameters of clear inhibitory rings were recorded to express antibacterial activity respectively. Results The immunized hemolymph possessed antibacterial activity against M.lysodeikticus after bathed at 100 ℃ for 1 min and also possessed antibacterial activity after bathed at 80 ℃ and 60 ℃ for 5 min. Conclusion The results imply that the induced antibacterial peptide of the blowfly can keep a certain stable antibacterial activity after treatment at high temperature.