Optimization of Ultrasonic-assisted Extraction of Chlorogenic Acid from Honeysuckle

In order to improve the extraction rate of Chlorogenic Acid from Honeysuckle,response surface methodology( RSM) was applied to optimize the extraction technology of Chlorogenic Acid from Honeysuckle. Based on single factor experiment,the influence factors of alcohol concentration、solution and material ration、 extraction temperature were studied on the contents of Chlorogenic Acid from Honeysuckle extraction. According to Box-Behnken combination method( Design-expert 8. 0. 5. 0),the optimal conditions were determined as follows: alcohol concentration 62% 、solution and material ration 18. 7∶ 1( mL / g) 、extraction temperature 58℃ 、 extraction time 40min. Under the conditions,the relative error between the testing values and predicted value was 3. 5%. This optimal extraction process can be used for extracting the Chlorogenic Acid in Honeysuckle. It is simple,stable and reproducible.