花青素
活性氧
超氧化物歧化酶
马维定
氧化应激
过氧化氢酶
化学
抗氧化剂
丙二醛
生物化学
谷胱甘肽过氧化物酶
谷胱甘肽
蛋白激酶B
活力测定
细胞凋亡
食品科学
氰化物
酶
作者
Wuyang Huang,W. Y. Han,Dajing Li,Jiangfeng Song,Yan Xiao,Chunquan Liu,Jing Zhou,Zhongquan Sui
标识
DOI:10.1021/acs.jafc.7b06135
摘要
Blueberry anthocyanins are considered protective of eye health because of their recognized antioxidant properties. In this study, blueberry anthocyanin extract (BAE), malvidin (Mv), malvidin-3-glucoside (Mv-3-glc), and malvidin-3-galactoside (Mv-3-gal) all reduced H2O2-induced oxidative stress by decreasing the levels of reactive oxygen species and malondialdehyde and increasing the levels of superoxide dismutase, catalase, and glutathione peroxidase in human retinal pigment epithelial cells. BAE and the anthocyanin standards enhanced cell viability from 63.69 ± 3.36 to 86.57 ± 6.92% (BAE), 115.72 ± 23.41% (Mv), 98.15 ± 9.39% (Mv-3-glc), and 127.97 ± 20.09% (Mv-3-gal) and significantly inhibited cell apoptosis (P < 0.01 for all). Mitogen-activated-protein-kinase pathways, including ERK1/2 and p38, were involved in the bioactivities. In addition, the anthocyanins decreased vascular-endothelial-cell-growth-factor levels and activated Akt-signal pathways. These combined results supported the hypothesis that blueberry anthocyanins could inhibit the induction and progression of age-related macular degeneration (AMD) through antioxidant mechanisms.
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