脂肪生成
生物
脂肪细胞
血小板源性生长因子受体
细胞生物学
PDGFRA公司
生长因子
受体
癌症研究
内分泌学
遗传学
脂肪组织
间质细胞
间充质干细胞
主旨
作者
Chengyi Sun,Hiromi Sakashita,Jang Kim,Zifeng Tang,G. Michael Upchurch,Longbiao Yao,William L. Berry,Timothy M. Griffin,Lorin E. Olson
出处
期刊:Cell Stem Cell
[Elsevier]
日期:2020-05-01
卷期号:26 (5): 707-721.e5
被引量:45
标识
DOI:10.1016/j.stem.2020.03.004
摘要
Adipocyte progenitors (APs) express platelet-derived growth factor receptors (PDGFRs), PDGFRα and PDGFRβ. Elevated PDGFRα signaling inhibits adipogenesis and promotes fibrosis; however, the function of PDGFRs in APs remains unclear. We combined lineage tracing and functional analyses in a sequential dual-recombinase approach that creates mosaic Pdgfr mutant cells by Cre/lox recombination with a linked Flp/frt reporter to track individual cell fates. Using mosaic lineage labeling, we show that adipocytes are derived from the Pdgfra lineage during postnatal growth and adulthood. In contrast, adipocytes are only derived from the mosaic Pdgfrb lineage during postnatal growth. Functionally, postnatal mosaic deletion of PDGFRα enhances adipogenesis and adult deletion enhances β3-adrenergic-receptor-induced beige adipocyte formation. Mosaic deletion of PDGFRβ also enhances white, brown, and beige adipogenesis. These data show that both PDGFRs are cell-autonomous inhibitors of adipocyte differentiation and implicate downregulation of PDGF signaling as a critical event in the transition from AP to adipocyte.
科研通智能强力驱动
Strongly Powered by AbleSci AI