生物正交化学
唾液酸
化学
生物化学
代谢途径
低聚糖
酶
点击化学
组合化学
作者
Zoeisha S. Chinoy,Clément Bodineau,Camille Favre,Kelley W. Moremen,Raúl V. Durán,Frédéric Friscourt
标识
DOI:10.1002/anie.201814266
摘要
Abstract The metabolic oligosaccharide engineering (MOE) strategy using unnatural sialic acids has recently enabled the visualization of the sialome in living systems. However, MOE only reports on global sialylation and dissected information regarding subsets of sialosides is missing. Described here is the synthesis and utilization of sialic acids modified with a sydnone reporter for the metabolic labeling of sialoconjugates. The positioning of the reporter on the sugar significantly altered its metabolic fate. Further in vitro enzymatic assays revealed that the 9‐modified neuraminic acid is preferentially accepted by the sialyltransferase ST6Gal‐I over ST3Gal‐IV, leading to the favored incorporation of the reporter into linkage‐specific α2,6‐ N ‐linked sialoproteins. This sydnone sugar presents the possibility of investigating the roles of specific sialosides.
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