Aiming at the problem of rolling bearing fault diagnosis, a fault diagnosis method of rolling bearing is proposed based on analytical mode decomposition (AMD) and LabVIEW. For the fault feature frequency of rolling bearing is predictable, the AMD method can be used to extract the signal in the frequency band of fault characteristic frequency in rolling bearing signal, and seek frequency spectrum of vibration signal. If the spectrum contains fault characteristic frequency, then the rolling bearing fault can be diagnosed by vibration signal. A rolling bearing fault diagnosis system is developed based on LabVIEW and AMD, and the application of AMD algorithm is realized. The validity of the method is proved by the analysis of actual fault signal of rolling bearing.