生物
植物菌原体
效应器
扫帚
叶状体
拟南芥
形态发生
毒力
拟南芥
植物
双分子荧光互补
细胞生物学
遗传学
突变体
基因
基因型
生态学
限制性片段长度多态性
作者
Peng Chen,Yu Zhang,Yonghua Li,Qiqi Yang,Qicheng Li,Li-Chuan Chen,Yun Chen,Ye Xia,Bin Tan,Xianbo Zheng,Jun Cheng,Wei Wang,Jidong Li,Jiancan Feng
出处
期刊:Phytopathology
[Scientific Societies]
日期:2023-07-12
被引量:1
标识
DOI:10.1094/phyto-10-21-0448-r
摘要
Plant-pathogenic phytoplasmas secrete specific virulence proteins into a host plant to modulate plant function for their own benefit. Identification of phytoplasmal effectors is a key step toward clarifying the pathogenic mechanisms of phytoplasma. In this study, Zaofeng3, also known as secreted Jujube Witches' broom phytoplasma protein 3 (SJP3), was a homologous effector of SAP54 and induced a variety of abnormal phenotypes, such as phyllody, malformed floral organs, witches' broom and dwarfism in Arabidopsis thaliana. Zaofeng3 can also induce small leaves, dwarfism and witches' broom in Ziziphus jujuba. Further experiments showed that the three complete alpha helix domains predicted in Zaofeng3 were essential for induction of disease symptoms in jujube. Yeast two-hybrid (Y2H) library screening showed that Zaofeng3 mainly interacts with proteins involved in flower morphogenesis and shoot proliferation. Bimolecular fluorescence complementation (BiFC) assays confirmed that Zaofeng3 interacted with these proteins in the whole cell. Overexpression of zaofeng3 in jujube shoot significantly altered the expression patterns of ZjMADS19, ZjMADS47, ZjMADS48, ZjMADS77 and ZjTCP7, suggesting that overexpressing zaofeng3 might induce floral organ malformation and witches' broom by altering the expression of the TFs involved in jujube morphogenesis.
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