生物制造
代谢工程
可持续生产
化学
生化工程
过程集成
生物生产
工业生物技术
合成生物学
计算生物学
Boosting(机器学习)
生物技术
基因
可扩展性
生物
工业微生物学
代谢途径
商品化学品
通量平衡分析
计算机科学
焊剂(冶金)
发酵
作者
Jiawei Meng,Yingying Zhu,Zhen Lu,Wenli Zhang,Wan Li,Tao Zhang,Wanmeng Mu
标识
DOI:10.1021/acssuschemeng.5c06685
摘要
l-Fucose, a functional monosaccharide with high commercial potential in pharmaceuticals, nutraceuticals, and cosmetics, can be efficiently produced via microbial synthesis. This study established a robust l-fucose production platform through a comprehensive genomic integration and pathway optimization. Key GDP-l-fucose biosynthetic genes were chromosomally integrated with optimized constitutive synthetic promoters, enhancing the precursor supply and yielding 6.47 g/L l-fucose. Copy number amplification of the α1,2-fucosyltransferase gene (BKHT) further improved flux through the “GDP-l-fucose → 2′-FL → l-fucose” pathway. Crucially, growth-phase-dependent expression of α-l-fucosidase (afcA) using the stationary-phase promoter alleviated metabolic burden, boosting titer to 15.37 g/L. Combinatorial cofactor engineering prioritized biomass formation, while fed-batch fermentation with delayed lactose induction achieved 86.58 g/L l-fucose in 95 h─without plasmids, inducers, or antibiotics. This integrated strategy establishes a scalable industrial process for sustainable l-fucose biomanufacturing.
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