聚电解质
化学
纳米反应器
催化作用
检出限
过氧化物酶
生物传感器
纳米技术
纳米颗粒
人工酶
组合化学
有机化学
材料科学
聚合物
色谱法
酶
生物化学
作者
Liang Sun,Zhinan Fu,Enguang Ma,Jiangtao Guo,Ziyu Zhang,Wenxin Li,Li Li,Zhiyong Liu,Xuhong Guo
出处
期刊:Langmuir
[American Chemical Society]
日期:2022-10-13
卷期号:38 (42): 12915-12923
被引量:11
标识
DOI:10.1021/acs.langmuir.2c02056
摘要
Distinct platinum (Pt) nanozymes as peroxidase mimics have received extensive interest owing to their outstanding catalytic activity, high environmental tolerance, lower consumption, and great potential in replacing natural enzymes. However, easy agglomeration of Pt nanoparticles (Pt NPs) resulting from the high surface free energy significantly decrease their peroxidase-like activity. Herein, spherical polyelectrolyte brush (SPB)-stabilized ultrasmall Pt NPs (SPB@Pt NPs) were prepared by a novel synthetic strategy where the SPB not only performed as a nanoreactor for the synthesis of ultrasmall Pt NPs but also greatly stabilized Pt NPs against aggregation. The well-defined SPB@Pt NP nanozymes exhibited outstanding peroxidase-like activity for the catalytic oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to blue oxidized TMB and were then used to establish a colorimetric sensor for rapid detection of cysteine, giving a limit of detection of 0.11 μM. Moreover, the colorimetric detection system was demonstrated with outstanding performance in sensitive and selective detection of cysteine in the presence of several interference molecules. From these results, SPB@Pt NPs have been regarded as promising peroxidase mimics for a large number of applications such as in biosensing, biomedicine, the food industry, and environmental chemistry.
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