How germination time affects protein hydrolysis of lupins during gastroduodenal digestion and generation of resistant bioactive peptides

Germination time is a critical factor that influences the digestibility and bioactivity of proteins in pulses. The objective was to understand the effect of sprouting time on protein hydrolysis (PH) and the release of bioactive peptides during digestion of lupin (Lupinus angustifolius L.) to provide recommendations on the optimum germination time for maximum nutritional and health benefits. Protein hydrolysis was monitored during germination and digestion by gel electrophoresis, size exclusion chromatography, and the analysis of soluble protein (SP), peptides (PEP), free amino acids (FAA) and free amino groups. The anti-inflammatory activity of intestinal digests was investigated in cell culture assays. Peptidomic and in silico analyses of intestinal digesta were conducted to identify digestion-resistant bioactive fragments. Germination time increased SP, PEP, and FAA. During digestion, the PH and release of small peptides was higher in sprouted lupin than control flour. Intestinal digests from sprouted lupin flour for 7 days exhibited the highest anti-inflammatory activity. In this sample, 11 potential bioactive peptides were identified. These findings open the exploration of novel food formulations based on sprouted lupins with higher protein digestibility and health-promoting potential.