DNA甲基化
甲基化
生物
亚硫酸氢盐测序
表观遗传学
遗传学
DNA
基因
基因表达
作者
Zhenzhen Xie,Songlin Sun,Honglei Ji,Maohua Miao,Wanhong He,Xiaoyan Song,Wencheng Cao,Qihan Wu,Hong Li,Wei Yuan
标识
DOI:10.1016/j.jhazmat.2023.132845
摘要
Epidemiological studies regarding the relationship between per- and polyfluoroalkyl substances (PFAS) and DNA methylation were limited. We investigated the associations of maternal PFAS concentrations with placental DNA methylation and examined the mediating role of methylation changes between PFAS and infant development. We measured the concentrations of 11 PFAS in maternal plasma during early pregnancy and infant development at six months of age. We analyzed genome-wide DNA methylation in 16 placental samples using reduced representation bisulfite sequencing. Additionally, we measured DNA methylation levels using bisulfite amplicon sequencing in 345 mother-infant pairs for five candidate genes, including carbohydrate sulfotransferase 7 (CHST7), fibroblast growth factor 13 (FGF13), insulin receptor substrate 4 (IRS4), paired like homeobox 2Ap (PHOX2A), and plexin domain containing 1 (PLXDC1). We found that placental DNA methylation profiles related to PFOA mainly enriched in angiogenesis and neuronal signaling pathways. PFOA was associated with hypomethylation of IRS4 and PLXDC1, and PFNA was associated with PLXDC1 hypomethylation. There were positive associations of CHST7 methylation with PFTrDA and IRS4 methylation with PFDoA and PFTrDA. PLXDC1 hypomethylation mediated the association between PFOA and suspected developmental delay in infants. Future studies with larger sample sizes are warranted to confirm these findings. Per- and polyfluoroalkyl substances (PFAS) are persistent organic pollutants that have been linked to adverse health outcomes in children. DNA methylation has been suggested as one of the underlying mechanisms. We reported for the first time the associations between several PFAS and DNA methylation of five candidate genes, in contrast to previous studies that mainly targeted DNA methylation in cord blood. We also noted the mediating role of plexin domain containing 1 (PLXDC1) methylation between PFAS and infant development. These findings highlight the importance of further exploring the involvement of placental DNA methylation in the health effects of PFAS.
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