Development and validation of RP-HPLC method for simultaneous estimation of rosuvastatin and bisoprolol fumarate in bulk and formulations

A simple, accurate and precise RP-HPLC method was developed for simultaneous estimation of Rosuvastatin and Bisoprolol fumarate in bulk and in formulation. Separation was achieved using and C18 column and Methanol: Phosphate buffer (pH 3.5) at a ratio of 45:55% V/V, flow rate was maintained at 1.0 ml. min and 245 nm was used as determination wavelength. System suitability parameters shows values within specified limits. Developed method was validated as per ICH guidelines and all the parameters were found to be with in the specified limits as per regulatory guidelines. Linearity was found to be 4-20 and 8-40 mcg/ml for Rosuvastatin and Bisoprolol fumarate respectively. Results of accuracy was found to be with in 98-102% which shows accuracy of the developed method.