清脆的
基因组编辑
Cas9
转化（遗传学）
生物
计算生物学
胡椒粉
基因组
核糖核酸
引导RNA
遗传学
病毒学
基因
食品科学
作者
Chenglu Zhao,Huanhuan Lou,Qian Liu,Siqi Pei,Qiansheng Liao,Zhenghe Li
摘要
Tomato spotted wilt virus-mediated delivery of CRISPR/Cas9 bypasses the need for stable transformation and permits efficient, DNA-free genome editing in pepper. Remarkably, up to 77.9% of regenerated pepper plants contained heritable edits. This method has been validated with two pepper varieties and is compatible with existing tissue culture protocols.
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