Abstract : The results testified that the process of L-form formation does not contribute to the removal of prophage in the lysogenic cultures studied by us. The preservation of prophage in L-forms, established on the basis of spontaneous and inductive production of phage, indicates that the interrelationship between prophage and the chromosome in L-forms is not disrupted, and that the sectors of the chromosome where prophage is localized does not undergo noticeable changes during the process of L-form formation. The preservation of lysogenicity in L- forms is an additional criterion of their genetic nature, and it also creates the prerequisites for studying their intimate genetic structure.