魚針草(Anisomelesindica)粗萃取物植物固醇β-穀固醇(β-sitosterol)與豆固醇(stigmasterol)之含量分析及相關藥理活性綜論

The phytosterol of Anisomeles indica: β-sitosterol, stigmasterol, at the presently research, people knew that there is not only an effect to lower the cholesterol but also reduce the cause rate of atherosclerosis. Besides, it orders some related of pharmacological activities from the related references, such as HepG2-cells, antiinflammatory effect, immunomodulatory activities, prostate cancer PC-3 cells, thyroid inhibiting, anti-osteoarthritic properties, etc. First, in this study, the phytosterol standard in the laboratory is used in the column for the purpose to analyze the phytosterol by HPLC. After the quantitative analysis, it gets the peak area rate of β-sitosterol and stigmasterol ( 3 : 4 ). Following the calculate, it gains the estimation of each content in 1mg phytosterol is β-sitosterol 0.04 % and stigmasterol 0.053 %. Next, it analyzes the quantification of stigmasterol and β-sitosterol which is extracted from different ways in the crude extract of Anisomeles indica by HPLC. The result is stigmasterol 0.029 % and β-sitosterol 0.045 % by chloroform; it can’t be analyzed the quantification of stigmasterol and β-sitosterol by water (included 5 % Ethanol) in the crude extract of Anisomeles indicaⅠand Ⅱ.