Estimation of Standard Melittin for Recombinant Melittin in Purified Bee Venom from(Apis mellifera L.) by Liquid Chromatography

A liquid chromatography method was developed for the determination of melittin content in purified bee venom as a traditional medicine possessing specific biological activity. Here, we investigated the possibility of using recombinant melittin instead of melittin isolated from honeybee venom as melittin standard. This result demonstrates that recombinant melittin is a suitable alternative as melittin standard to melittin isolated from honeybee venom. Thus, recombinant melittin is a cheap and sensitive alternative and can be applied to the measurement of melittin content in honeybee venom.