Self-assembly nanozymes combat diabetic wounds infected by multidrug-resistant bacteria

The effective treatment of diabetic wounds remains a significant clinical challenge, largely owing to the complex pathological microenvironment characterized by impaired angiogenesis, chronic inflammation, and delayed tissue repair. Therefore, developing advanced wound-healing strategies that can regulate the diabetic microenvironment and accelerate tissue regeneration is of great importance. In this work, we first constructed a Fe 2+ -induced self-assembled nanozymes through the coordination of Fe 2+ with the hydroxyl groups of epigallocatechin gallate (EGCG) and the amino groups of metformin (MET). Within this nanozyme, Fe 2+ catalyzes Fenton reactions to generate reactive oxygen species (ROS) for efficient antibacterial activity, while EGCG disrupts bacterial membranes, and MET lowers blood glucose levels to modulate the hyperglycemic wound environment. The synergistic antibacterial and metabolic regulatory effects significantly suppressed bacterial infection, alleviated oxidative stress, and promoted angiogenesis and collagen deposition in diabetic wound models. Both in vitro and in vivo evaluations demonstrate that the multifunctional nanozymes effectively accelerated wound healing and tissue regeneration. This work provides a facile and green self-assembly strategy for constructing multifunctional therapeutic systems and offers a promising and clinically translatable approach for the treatment of diabetic and other chronic wounds.