Living single-cell metabolomics via mass spectrometry: state of the art and perspective

chemical derivatizations, AI-powered data processing pipelines, and integrated multi-omics analyses at the single-cell level. Despite existing hurdles, continuous progress in technology, data science, and interdisciplinary collaboration is expected to bring transformative breakthroughs in MS-based living SCM, ultimately advancing our understanding of dynamic biological processes and accelerating biomedical discovery.