川地34
祖细胞
骨髓
干细胞
肥大细胞
造血
诱导多能干细胞
细胞生物学
生物
免疫学
化学
分子生物学
病理
医学
胚胎干细胞
生物化学
基因
作者
Arnold S. Kirshenbaum,Dean D. Metcalfe
出处
期刊:Humana Press eBooks
[Humana Press]
日期:2005-08-18
卷期号:: 105-112
被引量:24
标识
DOI:10.1385/1-59259-967-2:105
摘要
Human mast cells (HMCs) are derived from a CD34+ pluripotent progenitor cell that is Kit (CD117+), CD13+, Fc epsilonRI- and lacks lineage-specific surface markers. Bone marrow and peripheral blood are two tissue sources available for obtaining CD34+ progenitor cells from which to culture HMCs. CD34+ cells can be isolated and enriched by magnetic separation columns and stored under specific conditions until ready for use. Alternatively, enriched CD34+ cells may be immediately cultured in serum-free culture media containing recombinant human stem cell factor (rhSCF), rhIL-6, and rhIL-3 (first week only). Weekly hemidepletions and the removal of adherent cells and/or debris enables the investigator to obtain HMC cultures, identified by Wright-Giemsa and acidic toluidine blue stains, by 8-10 wk.
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