化学
纳米尺度
纳米技术
制作
医学
材料科学
替代医学
病理
作者
Yasufumi Takahashi,Yuya Sasaki,Takeshi Yoshida,Kota Honda,Yifei Zhou,Takafumi Miyamoto,Tomoko Motoo,Hideyuki Higashi,Andrew Shevchuk,Yuri Korchev,Hiroki Ida,Rikinari Hanayama,Takeshi Fukuma
标识
DOI:10.1021/acs.analchem.3c01010
摘要
Scanning ion conductance microscopy (SICM) is a promising tool for visualizing the dynamics of nanoscale cell surface topography. However, there are still no guidelines for fabricating nanopipettes with ideal shape consisting of small apertures and thin glass walls. Therefore, most of the SICM imaging has been at a standstill at the submicron scale. In this study, we established a simple and highly reproducible method for the fabrication of nanopipettes with sub-20 nm apertures. To validate the improvement in the spatial resolution, we performed time-lapse imaging of the formation and disappearance of endocytic pits as a model of nanoscale time-lapse topographic imaging. We have also successfully imaged the localization of the hot spot and the released extracellular vesicles. The nanopipette fabrication guidelines for the SICM nanoscale topographic imaging can be an essential tool for understanding cell-cell communication.
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