Nitrogen fertilizer builds soil organic carbon under straw return mainly via microbial necromass formation

Carbon (C) and nitrogen (N) inputs strongly influence the formation, turnover and sequestration of soil organic carbon (SOC) in agricultural ecosystems. It is not clear, however, how N input regulates the contribution of plant- and microbial-derived C to SOC sequestration under straw return. To fill this gap, plant and microbial biomarkers, as well as enzyme activities were determined in a long-term (18 years) field experiment. Straw return and N fertilization increased SOC content by 20% and 10%, respectively. Specifically, straw return increased the proportion of total lignin (mainly vanillyl and syringyl) phenols in SOC by 16%, but decreased the proportion of cinnamyl in SOC by 7.5%. This implied that some plant residues were selectively preserved, while the compounds that were less stable than cinnamyl were easily decomposed. The increased phospholipid fatty acid (PLFA) content and enzyme activities with straw return indicated the acceleration of straw decomposition. Based on amino sugar content, straw return did not alter the proportion of microbial necromass to SOC. Together, lignin and amino sugars co-determined the stable contribution of plant- and microbial-derived C to SOC sequestration under straw return. N fertilization increased the portion of microbial necromass (especially bacterial necromass) C in SOC by 6% and thus decreased the plant residue contribution to SOC. Accordingly, N fertilization accelerated the microbial utilization of straw and consequently microbial necromass formation. In terms of PLFA composition, Ascomycota and Basidiomycota, Actinobacteria, and Gram-negative bacteria were the key groups forming microbial necromass and thus SOC. N fertilization increased N-acquiring enzyme activities and boosted the involvement of microbial necromass in nutrient cycling, which in turn may stimulate plant and microbial growth. Overall, straw return simultaneously increased plant- and microbial-derived C, while N fertilization stimulated microbial growth and enzyme activity and thus increased straw conversion to microbial necromass.