Facile One‐Pot Assembly of a Versatile Photoactive Nanozyme for Highly Efficient Antibacterial Therapy

Abstract Conventional combinational antibacterial therapy requires the complicated assembly of multiple components that might cause a premature leak of therapeutic agents. Thus the one‐pot assembly of highly integrated multifunctional antibacterial agents is highly desirable for treating multidrug‐resistant (MDR) bacteria. Herein, the vancomycin‐derived carbon dots (Van‐CDs) are facilely developed as a compact and powerful multifunctional photodynamic nanozyme platform that achieves the augmented reactive oxygen species (ROS) generation for accelerating bacterial elimination. By residual recognition groups of vancomycin, the red emissive Van‐CDs gain a specific affinity toward bacteria with a high binding constant of 20 L g −1 , manifesting a superior bacteria‐imaging ability. Meanwhile, Van‐CDs are encoded with an excellent peroxidase‐mimicking (POD) activity for mediating the H 2 O 2 ‐activated evolvement of •OH, and also endowed with intrinsic photodynamic property for simultaneously producing singlet oxygen ( 1 O 2 ). Surprisingly, the photodynamic Van‐CDs nanozyme possesses an auxiliary photothermal feature, which enables the photothermal imaging‐guided hyperthermia‐reinforced antibacterial therapy in vivo. Furthermore, a subcutaneous abscess model is established to validate their pronounced biofilm eradication and wound healing acceleration in vivo through the Van‐CDs‐promoted collagen deposition. Taken together, the present all‐in‐one compact photodynamic carbon‐derived nanozyme with multiple intrinsic therapeutic functions represents a promising and competitive candidate for treating diabetic infections of clinical research.