化学
尿素酶
纳米团簇
生物传感器
制作
纳米技术
盐(化学)
组合化学
有机化学
酶
生物化学
医学
材料科学
替代医学
病理
作者
Yaqing Han,Mengke Wang,Hui Xie,Yitong Zhou,Shun Wang,Guannan Wang
出处
期刊:Talanta
[Elsevier]
日期:2024-01-01
卷期号:: 125725-125725
标识
DOI:10.1016/j.talanta.2024.125725
摘要
Sensitive and convenient sensing of urease and its inhibitors is exceptionally urgent in clinical diagnosis and new drug development. In this study, the gold nanoclusters (AuNCs) and hydroxyl double salt (HDS) were composited by a simple confinement effect to prepare highly fluorescent AuNCs@HDS composites to monitor urease and its drug inhibitors. HDS was used as a matrix to confine AuNCs (AuNCs@HDS), facilitating the emission intensity of AuNCs. However, acidic conditions (low pH) can disrupt the structure of HDS to break the confinement effect, and quench the fluorescence of AuNCs. Therefore, a sensing platform for pH-related enzyme urease detection was constructed based on the sensitive response of AuNCs@HDS to pH. This sensing platform had a linear response range of 0.5–22.5 U/L and a low limit of detection (LOD) of 0.19 U/L for urease. Moreover, this sensing platform was also applied to monitor urease inhibitors and urease in human saliva samples. Additionally, a portable hydrogel kit combined with a smartphone was developed for urease detection to achieve portable, low-cost, instrument-free, and on-site monitoring of urease.
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