生物
细胞生物学
分生组织
拟南芥
细胞分裂
电池极性
GTP酶
CDC42型
Notch信号通路
突变体
串扰
遗传学
信号转导
细胞
基因
物理
光学
作者
Duoyan Rong,Shuai Zhao,Wenxin Tang,Nan Luo,Hai He,Zhuqing Wang,Hui Ma,Yimei Huang,Xiaoxun Yao,Xue Pan,Lin Lv,Jiajing Xiao,Renyi Liu,Shingo Nagawa,Chizuko Yamamuro
标识
DOI:10.1073/pnas.2117803119
摘要
The formation of cell polarity is essential for many developmental processes such as polar cell growth and spatial patterning of cell division. A plant-specific ROP (Rho-like GTPases from Plants) subfamily of conserved Rho GTPase plays a crucial role in the regulation of cell polarity. However, the functional study of ROPs in angiosperm is challenging because of their functional redundancy. The Marchantia polymorpha genome encodes a single ROP gene, MpROP, providing an excellent genetic system to study ROP-dependent signaling pathways. Mprop knockout mutants exhibited rhizoid growth defects, and MpROP was localized at the tip of elongating rhizoids, establishing a role for MpROP in the control of polar cell growth and its functional conservation in plants. Furthermore, the Mprop knockout mutant showed defects in the formation of meristem notches associated with disorganized cell division patterns. These results reveal a critical function of MpROP in the regulation of plant development. Interestingly, these phenotypes were complemented not only by MpROP but also Arabidopsis AtROP2, supporting the conservation of ROP's function among land plants. Our results demonstrate a great potential for M. polymorpha as a powerful genetic system for functional and mechanistic elucidation of ROP signaling pathways during plant development.
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