This paper uses 8-bit high-performance AVR microcontroller ATmega16 as the core to make data acquisition card based on the AVR USB technology,which is described as a joystick.Then data acquisition can be realized on LabVIEW platform by calling this equipment,which has a higher transmission speed than traditional serial device.What′s more,it owns low cost and is easy to be realized.The experimental results showed that this data acquisition card can accomplish data acquisition on LabVIEW steadily.