Effects of Active Compounds from Shenmai on the Proliferation and Migration of VascularEndothelial Cell

Objective: To observe the effects of total saponins and total polysaccharides extracted from shenmai on the proliferation and migration of vascularendothelial cell.Methods: The effects of total saponins and total polysaccharides from shenmai on the proliferation of ECV-304 cell and gastric carcinoma SGC-7901 as well as ECV-304 cell induced by SGC-7901 cell were measured by MTT colorimetric assay.Their effects on the migration of ECV-304 cell were investigated by agaros assay.Results: When the concentration of total polysaccharides from shenmai was in the range of 0.18～2.88mg/mL,there was no influence on the proliferation of ECV-304 cell and gastric carcinoma SGC-7901 as well as ECV-304 cell induced by SGC-7901 cell when the concentration of total saponins from shenmai was in the range of 0.05～0.8mg/mL,there was no influence on the proliferation of(SGC-)7901 cell,but they were able to inhibit the proliferation of ECV-304 cell and ECV-304 cell induced by SGC-7901 cell.The inhibition was(13.16)%～62.77%.There was no influence of total polysaccharides from shenmai on the migration of ECV-304 cell,but total saponins from shenmai can inhibit the migration of ECV-304 cell.The inhibition was 42%～80%.The compounds of total saponins and total asragalans from shenmai can also inhibit the proliferation and migration of vascularendothelial cell,there were no difference between the compounds and total polysaccharides in statistics.Conclusion:Total saponins from shenmai can inhibit the proliferation of vascularendothelial cell and vascularendothelial cell induced by conditional cultured medium of gastric carcinoma cell,they also can inhibit the migration of vascularendothelial cell.Total saponins from shenmai are active compounds.