Inhibitory effect of microRNA-29c on proliferation and invasion of human prostate cancer cell line LNCaP

Objective To observe the effect of microRNA-29c on the proliferation and invasion abilities of human prostate cancer cell line LNCaP,and to discuss its potential application.Methods The difference in miR-29c expression between ADPC(androgen-dependent prostate cancer) and AIPC(androgen-independent prostate cancer) cell lines was observed by real time RT-PCR.MiR-29c-inhibitor(anti-miR-29c)was used to decrease miR-29c expression in LNCaP cells;then the cell proliferation was examined with CCK-8 assay,the cell cycle was analyzed using flow cytometry,and the invasive abilities of LNCaP cells were observed by transwell invasion assay before and after treatment with anti-miR-29c.Results The result of real-time RT-PCR showed that miR-29c expression in LNCaP-AI cells was significantly lower than that in LNCaP cells(P0.05).Down-expression of miR-29c in LNCaP cells significantly promoted the proliferation and invasion abilities of LNCaP cells(P0.05).Conclusion Normal expression of miR-29c plays an inhibitory role in the proliferation and invasion of LNCaP cells,indicating it might be a new target for biotherapy of prostate cancer.