Objective To investigate the effect of flavonoids of tatary buckwheat on serum lipids. Methods The hyperlipaemia animal model was established according to the reference. The mice and rats were randomly divided into control group and experimental group. The contents of total cholesterol (TC), triglyceride(TG), high density lipoprotein-cholesterol (HDL-C), hepatic triglyceride and blood glucose were determined. Results Flavonoids of tatary buckwheat reduced the level of TG of hyperlipaemic mice and the levels of TC and TG of hyperlipaemic rats significantly. But no effect was found on the content of HDL-C. Conclusion Tatary buckwheat can lower the level of serum lipids.