THE COMPARISION OF STUDY BETWEEN CISPLATIN AND CARBOPLATIN IN CHEMOTHERAPY OF CERVICAL CANCER

Objective:To evaluate the effect of cisplatin and carboplatin using chemosensitivity testing,and compare their cell toxicity and provide experimental evidence for clinical application.Methods:1.The mensuration of ATP standard curve.2.An ATP-CVA test was used to determine the drug sensitivity of Hela cells、Caski cells、Siha cells.The drug were cisplatin and carboplatin and the concentration were 0.5×PPC,1×PPC and 2×PPC respectively.Results:1.In a range of 10~(-9)-10~(-5) mol/ml,there is a linear relationship between the log ATP and the log luminescence(Y=0.892X+10.257,correlation coefficient r=0.998)(P0.001).2.At the concentration of 0.5×PPC、1×PPC and 2×PPC,the restraint rate of cisplatin of Hela cells were 53.5%、69.2% and 100% respectively,the restraint rate of carboplatin of Hela cells were 56.1%、64.2% and 98% respectively;the restraint rate of cisplatin of Siha cells were 78.3%、84% and 95% repectively,the restraint rate of carboplatin of Siha cells were 69.5%、77.8% and 98% respectively;the restraint rate of cisplatin of Caski cells were 36%、45% and 52.6%,respectively,the restraint rate of carboplatin of Caski cells were 40%、41.7% and 42.3%,respectively;the differences were not significant(P0.05).Conclusion:The difference of cell toxicity between cisplatin and carboplatin was not significant,and carboplatin is better than cisplatin in the chemotherapy of cervical cancer for its little side effect.