Expression of recombinant BPI_(23)-Fcγ1 fusion protein in E. coli

Objective To express BPI 23 Fcγ1 anti bacteria recombinant fusion protein. Methods BPI 23 and Fcγ1 genes were isolated by RT PCR from HL 60 and normal human leukocytes and inserted into expression vector pBV and the fusion protein expressed in E. coli DH5α in a temperature induced manner. Results The expressed BPI 23 Fcγ1 fusion protein constituted 20%～30% of total bacterial protein. The renatured BPI 23 Fcγ1 recombinant protein showed biological activities of anti bacteria and was able to form conjugate with protein A. Conclusion pBV BPI 600 Fcγ1 700 recombinant expression vector was successfully constructed, and BPI 23 Fcγ1 recombinant protein with anti bacteria activities was expressed in E. coli .